Evaluation of the semen quality of patients attended in a clinical analyzes in the Cascavel municipality, Brazil
Danieli Webber dos Reis, Claudinei Mesquita da Silva, Heloise Skiavine Madeira, Veridiana Lenartovicz Boeira, Denise Michele Indras, Jorge Juarez Vieira Teixeira, Leyde Daiane de Peder
Abstract
Objectives: The objective of this study was to investigate the prevalence of altered spermograms of individuals living in a municipality in the west of Paraná and attended at a private laboratory for clinical analysis, as well as the main alterations found in this study. Methods: We analyzed electronic reports of patients undergoing sperm examination in a private laboratory located in the municipality of Cascavel, Paraná, from January 2014 to December 2016. Results: It was verified that of 888 tests analyzed, only 1% presented all parameters analyzed within normality, while 99% of the cases presented some type of alteration. There was a change in the following parameters with statistical significance between the number of reports analyzed: time of liquefaction, sperm concentration, head anomalies, tail abnormalities, number of spermatocytes and spermatids (p <0.0001), viscosity (p = 0.1098), live forms (p = 0.0524), progressive motility (p = 0.0010) and total motility (p = 0.0013). Conclusions: There was a high prevalence of altered spermograms and a great number of alterations were observed, which requires the need for complementary tests to determine the causes of these alterations.
Keywords
References
1. Practice Committee of the American Society for Reproductive Medicine. Definitions of infertility and recurrent pregnancy loss. Fertil Steril. 2008;89(6):1603. http://dx.doi.org/10.1016/j.fertnstert.2008.03.002. PMid:18485348.
2. Cruz JC. Factores genéticos na infertilidade masculina [dissertação]. Viana do Castelo: Faculdade de Medicina, Universidade do Porto; 2010. 29 p.
3. Stirnemann JJ, Samson A, Bernard JP, Thalabard JC. Day-specific probabilities of conception in fertile cycles resulting in spontaneous pregnancies. Hum Reprod. 2013;28(4):1110-6. http://dx.doi.org/10.1093/humrep/des449. PMid:23340057.
4. Boivin J, Bunting L, Collins AJ, Nygren KG. International estimates of infertility prevalence and treatment seeking: potencial need and demand for infertility medical care. Hum Reprod. 2007;22(6):1506-12. http://dx.doi.org/10.1093/humrep/dem046. PMid:17376819.
5. Wilkes S, Chinn DJ, Murdoch A, Rubin G. Epidemiology and management of infertility: a population based study in UK primary care. Fam Pract. 2009;26(4):269-74. http://dx.doi.org/10.1093/fampra/cmp029. PMid:19502575.
6. Pasqualotto FF. Investigação e reprodução assistida no tratamento da infertilidade masculina. Rev Bras Ginecol Obstet. 2007;29(2):103-12. http://dx.doi.org/10.1590/S0100-72032007000200008.
7. Mascarenhas MN, Flaxman SR, Boerma T, Vanderpoel S, Stevens GA. National, regional, and global trends in infertility prevalence since 1990: a systematic analysis of 277 health surveys. PLoS Med. 2012;9(12):e1001356. http://dx.doi.org/10.1371/journal.pmed.1001356. PMid:23271957.
8. Lipshultz LI, Howards SS, Niederberger CS. Infertility in the male. Cambridge: Cambridge University Pres; 2009. http://dx.doi.org/10.1017/CBO9780511635656.
9. Dohle GR, Diemer T, Giwercm A, Jungwirth A, Kopa Z, Krausz C. Male infertility. Hakobyan: European Association of Urology; 2010.
10. Santos GC. Prevalência de microdeleções nas regiões AZFa, AZFb e AZFc do cromossomo Y em indivíduos com oligozoospermia ou azoospermia em Mato Grosso [dissertação]. Cuiabá: Universidade Federal do Mato Grosso; 2011. 59 p.
11. World Health Organization. Laboratory manual for the examination and processing of human semen. Geneva: WHO; 2010.
12. Sloter E, Schmid TE, Marchetti F, Eskenazi B, Nath J, Wyrobek AJ. Quantitative effects of male age on sperm motion. Hum Reprod. 2006;21(11):2868-75. http://dx.doi.org/10.1093/humrep/del250. PMid:16793993.
13. Nallella KP, Sharma RK, Aziz N, Agarwal A. Significance of sperm characteristics in the evaluation of male infertility. Fertil Steril. 2006;85(3):629-34. http://dx.doi.org/10.1016/j.fertnstert.2005.08.024. PMid:16500330.
14. Elzanaty S, Malm J, Giwercman A. Duration of sexual abstinence: epididymal and accessory sex gland secretions and their relationship to sperm motility. Hum Reprod. 2005;20(1):221-5. http://dx.doi.org/10.1093/humrep/deh586. PMid:15550495.
15. Gonçalves J. Avaliação do casal infértil. Rev Port Clin Geral. 2005;21(5):493-503.
16. Barbosa FFS. Influência dos antioxidantes na qualidade do sêmen de homens em tratamento de fertilidade [dissertação]. Lisboa: Faculdade de Ciências, Universidade de Lisboa; 2009. 70 p.
17. Pasqualotto FF, Fonseca GP, Silva ML, Ferreira RV, Zago BE, Garbin C Jr, et al. Influência do período de abstinência sexual sobre as características seminais de homens inférteis. Rev Bras Ginecol Obstet. 2006;28(1):4449. http://dx.doi.org/10.1590/S0100-72032006000100008.
18. Lima VM, Barbieri RL, Aquila AD, Myamora F, Duarte IS. Estudo histopatológico do efeito da nicotina nos testículos e epidídimos de ratos. Rev Bras Clin Med. 2012;10(4):322-8.
19. Zhu QX, Meads C, Lu ML, Wu JQ, Zhou WJ, Gao ES. Turning point of age for semen quality: a population-based study in Chinese men. Fertil Steril. 2011;96(3):572-6. http://dx.doi.org/10.1016/j.fertnstert.2011.06.058. PMid:21782163.
20. Du Plessis SS, Cabler S, Mcalister DA, Sabanegh E, Agarwal A. The effect of obesity on sperm disorders and male infertility. Nat Rev Urol. 2010;7(3):153-61. http://dx.doi.org/10.1038/nrurol.2010.6. PMid:20157305.
21. Hazout A, Dumont-Hassan M, Junca A-M, Bacrie PC, Tesarik J. High magnification ICSI overcomes paternal effect resistant to conventional ICSI. Reprod Biomed Online. 2006;12(1):19-25. http://dx.doi.org/10.1016/S1472-6483(10)60975-3. PMid:16454928.
22. Vagnini L, Baruffi RL, Mauri AL, Petersen CG, Massaro FC, Pontes A, et al. The effects of male age on sperm DNA damage in an infertile population. Reprod Biomed Online. 2007;15(5):514-9. http://dx.doi.org/10.1016/S1472-6483(10)60382-3. PMid:18028741.
23. Borini A, Tarozzi N, Bizzaro D, Bonu MA, Fava L, Flamigni C, et al. Sperm DNA Fragmentation: paternal effect on early postimplantation embryo development in ART. Hum Reprod. 2006;21(11):2876-81. http://dx.doi.org/10.1093/humrep/del251. PMid:16793992.
24. Kleinhaus K, Perrin M, Friedlander Y, Paltiel O, Malaspina D, Harlap S. Paternal age and spontaneous abortion. Obstet Gynecol. 2006;108(2):369-77. http://dx.doi.org/10.1097/01.AOG.0000224606.26514.3a. PMid:16880308.
25. Lackner J, Schatzl G, Horvath S, Kratzik C, Marberger M. Value of counting white blood cells (WCB) in semen samples to predit the presence of bacteria. Eur Urol. 2006;49(1):148-52, discussion 152-3. http://dx.doi.org/10.1016/j.eururo.2005.08.017. PMid:16314032.
26. Piva S. Espermograma – Análise e técnicas. 6. ed. Santos; 1985.
27. Brahem S, Mehdi M, Elghezal H, Saad A. The effects of male aging on semen quality, sperm DNA fragmentation and chromosomal abnormalities in an infertile population. J Assist Reprod Genet. 2011;28(5):425-32. http://dx.doi.org/10.1007/s10815-011-9537-5. PMid:21287403.
28. Berling S, Wolner-Hanssen P. No evidence of deteriorating semen quality among men in infertile relationships during the last decade: a study of males from Southern Sweden. Hum Reprod. 1997;12(5):1002-5. http://dx.doi.org/10.1093/humrep/12.5.1002. PMid:9194654.
29. Wyrobek AJ, Eskenazi B, Young S, Arnheim N, Tiemann-Boege I, Jabs EW, et al. Advancing age has differential effects on DNA damage, chromatin integrity, gene mutations, and aneuploidies in sperm. Proc Natl Acad Sci USA. 2006;103(25):9601-6. http://dx.doi.org/10.1073/pnas.0506468103. PMid:16766665.
30. Jeyendran RS. Protocols for semen analysis in clinical diagnosis. 1st ed. New York: Parthenon Publishing; 2003.
31. Fisch H, Hyun G, Golden R, Hensle TW, Olsson CA, Liberson GL. The influence of paternal age on down syndrome. J Urol. 2003;169(6):2275-8. http://dx.doi.org/10.1097/01.ju.0000067958.36077.d8. PMid:12771769.
32. Garolla A, Fortini D, Menegazzo M, De Toni L, Nicoletti V, Moretti A, et al. High-power microscopy for selecting spermatozoa for ICSI by physiological status. Reprod Biomed Online. 2008;17(5):610-6. http://dx.doi.org/10.1016/S1472-6483(10)60307-0. PMid:18983744.
33. Bartoov B, Berkovitz A, Eltes F, Kogosovsky A, Yagoda A, Lederman H, et al. Pregnancy rates are higher with intracytoplasmic morphologically selected sperm injection than with conventional intracytoplasmic injection. Fertil Steril. 2003;80(6):1413-9. http://dx.doi.org/10.1016/j.fertnstert.2003.05.016. PMid:14667877.
34. American Society for Reproductive Medicine. Diagnostic evaluation of the infertile male: a committee opinion. Fertil Steril. 2015;103(3):18-25. http://dx.doi.org/10.1016/j.fertnstert.2014.12.103. PMid:25597249
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